Technical Reference #1932

1932.

Sarcomere Mechanics in Capillary Endothelial Cells Robert J. Russell; Shen-Ling Xia; Richard B. Dickinson; and Tanmay P. Lele, University of Florida, Biophysical Journal, 97(1932), (2009)
Link To Paper

Materials & Methods:
For experiments cells were transiently transfected with EGFP-a-actinin plasmid (kindly provided by Prof. Carol Otey) or EGFP-actin using the Effectene (Qiagen Valencia CA) reagent. Previous studies have shown that the fusion construct is functional and localizes with the endogenous protein (30). Cells were transfected for 6 h in a 12-well cell culture dish. After 24 h the cells were trypsinized (Gibco) and plated sparsely on glass bottom dishes (MatTek Ashland TX). For imaging experiments the media was changed to a CO2-independent media as described previously (31).

Microscopic Technique
Inverted laser scanning confocal microscope

Cell Type(s)
Bovine capillary endothelial (BCE) cells