Technical Reference #1925

1925.

Histone Crosstalk between H3S10ph and H4K16ac Generates a Histone Code that Mediates Transcription Elongation Alessio Zippo; Riccardo Serafini; Marina Rocchigiani; Susanna Pennacchini; Anna Krepelova; and Salvatore Oliviero, Universita di Siena, Cell, 138(1925), (2009)

Materials & Methods:
FRAP was carried out using the Leica TCS SP2 confocal microscope on living cells grew in glass-bottom petri dishes (MatTek Cultureware). Bleaching was performed with two 208 ms pulses using the 458 nm line of the Argon laser at 95% power on a 2 mmarea. Fluorescent recovery was monitored by collecting 120 images at 1 s intervals with low laser intensity (4%power with 458 laser line detection 465-510). Data were collected from at least three independent experiments and were normalized as previously described (Agresti et al. 2005).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
3T3 cells