Technical Reference #1920

1920.

Structural Characterization of Edematous Corneas by Forward and Backward Second Harmonic Generation Imaging Chiu-Mei Hsueh; Wen Lo; Wei-Liang Chen; Vladimir A. Hovhannisyan; Guang-Yu Liu; Sheng-Shun Wang; Hsin-Yuan Tan; and Chen-Yuan Dong, National Taiwan University, Biophysical Journal, 97(1920), (2009)
Link To Paper

Materials & Methods:
The bovine corneal buttons of these two groups were placed on a glass bottom culture dish (Part No. P50G-1.5-14-F; MatTek Ashland MA) and then covered with coverglasses (No. 990; Mikroskopische Deckglaser Karl Hecht KG Assistent Sondheim Germany) to maintain corneal moisture. Each corneal button was imaged in the direction from the anterior to the posterior stroma (forward direction) and imaged again in the direction from the posterior to the anterior stroma (reverse direction) by inverting the culture dish.

Microscopic Technique
SHG Microscopy

Cell Type(s)
bovine corneas