Technical Reference #1911

1911.

TCP-FA4: A derivative of tranylcypromine showing improved blood-brain permeability Kelly E. Desino; Rosario Pignatello; Salvatore Guccione; Livia Basile; Sabah Ansar; Mary Lou Michaelis; Rona R. Ramsay; Kenneth L. Audus, University of Kansas, Biochemical Pharmacology, 78(1911), (2009)

Keywords:
Fatty acid chains; Lipoamino acids; Blood-brain barrier (BBB); Neuroprotection; Beta-Amyloid; Monoamine oxidase inhibition

Materials & Methods:
Dissociated cortical cell cultures were established from embryonic day 18 rat fetuses recovered from pregnant Sprague– Dawley rats (Harlan Sprague Dawley Inc. Indianapolis IN) as described previously [18]. After the final precipitation step neurons were suspended in fresh Dulbecco’s modified Eagle’s medium/F-12 with 10% fetal bovine serum (Atlanta Biologicals Lawrenceville GA) and plated at a density of 2.5 105 cells in 35 mm glass bottom microwell dishes (MatTek Co. Ashland MA) coated with poly-D-lysine. Serum-containing medium was removed after 24 h and the cells were maintained in serum-free Dulbecco’s modified Eagle’s medium/F-12 containing the N2 supplements. Cultures were grown at 37 8C in 5% CO2 and 97% humidity as previously described [18].

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Cortical cells