Technical Reference #190

190.

Importance Of Protein Kinase C Targeting For The Phosphorylation Of Its Substrate; Myristoylated Alanine-Rich C-Kinase Substrate Ohmori; S.; Sakai; N.; Shirai; Y.; Yamamoto; H.; Miyamoto; E.; Shimizu; N.; Saito ; N.;, Kobe University, The Journal of Biological Chemistry, 275(190), (2000)
Link To Paper

Abstract:
We visualized the translocation of myristoylated alanine-rich protein kinase C substrate (MARCKS) in living Chinese hamster ovary-K1 cells using MARCKS tagged to green fluorescent protein (MARCKS-GFP). MARCKS-GFP was rapidly translocated from the plasma m

Microscopic Technique
Confocal Microscopy, Laser Scanning, Fluorescence Microscopy

Cell Type(s)
CHO-K1