Technical Reference #1895

1895.

IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion Vasilena Gocheva, Hao-Wei Wang, Bedrick B. Gadea, Tanaya Shree, Karen E. Hunter, Alfred L. Garfall, Tara Berman, and Johanna A. Joyce, Memorial Sloan-Settering Cancer Center, Genes & Development, 24(1895), (2010)
Link To Paper

Keywords:
Tumor-associated macrophage; Tumor microenvironment; Cysteine Cathepsin; Protease; Interleukin-4; Invasion

Materials & Methods:
3D invasion assay - Primary macrophages were derived as above. Invasion of cancercells through a 3D collagen I matrix was performed as described previously (Goswami et al. 2005). Briefly, 80,000 MTLn3 cells were plated alone or in the presence of 200,000 BMDMs in 35-mm MatTek dishes. A collagen I matrix (BD Pharmingen) was added over cells 24 h after plating. Cells were allowed to migrate through the collagen I matrix for 24 h, and the number of cancer cells migrating $15 mm was quantified using confocal microscopy. Data were collected from at least three independent experiments performed in triplicate.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Primary macrophages