Technical Reference #1894

1894.

Lipoprotein interactions with chromatic membranes as a novel marker for oxidative stress-related diseases Nirit Hanin-Avraham; Bianca Fuhrman; Agnieszka Mech-Dorosz; Sofiya Klusheva; Angel Progador; Michael Aviram; Raz Jelinek, Ben Gurion University of the Negev, Biochimica et Biophysica Acta, 1788(1894), (2009)

Materials & Methods:
Cell samples for microscopy analysis were prepared according to the procedure described above (chromatic cell experiments) except that the cells were seeded on 35-mm glass-bottom MatTek dishes. Fluorescence images of the PDA-labeled cells were acquired on a laser-scanning confocal microscope Axiovert-100M (Zeiss Germany) with a Plan-Neofluar 63×/1.2 oil objective. Excitation wavelength 488 nm was produced by an argon laser. Emitted light was passed through an LP 505 nm filter.

Microscopic Technique
Confocal fluorescence microscopy

Cell Type(s)
Macrophages J-774