Technical Reference #1890

1890.

Subcellular localization of flavonol aglycone in hepatocytes visualized by confocal laser scanning fluorescence microscope Rie Mukai Æ Yasuhito Shirai Æ Naoaki Saito Æ Ken-ichi Yoshida Æ Hitoshi Ashida, Kobe University, Cytotechnology, 59(1890), (2009)
Link To Paper

Keywords:
Cellular uptake Flavonoid Fluorescence microscope Hepatocytes Kaempferol

Materials & Methods:
[3H]-Kaempferol was obtained from Moravek Biochemicals Inc. (Brea CA) flavone morin and naringenin from Nacalai tesque (Kyoto Japan) isorhamnetin kaempferol-3-glucoside kaempferol- 3-rutinoside quercetin apigenin luteolin galangin and eriodictyol from Extrasynthe`se (Genay France) kaempferol and rutin from Wako Pure Chemical Industries Ltd (Osaka Japan) and (-)-epigallocatechin (EGC) and (-)-Epigallocatechin gallate (EGCG) from Kurita Kogyo (Tokyo Japan). (-)-Epigallocatechin 3-(300-O-methyl) gallate (Met- EGCG) was generously provided by Dr. Ryoyasu Saijo. Propidium iodide was purchased from Molecular Probes Inc. (Carlsbad CA).

Microscopic Technique
Fluorescence

Cell Type(s)
Hepa-1c1c7