Technical Reference #1883

1883.

A Novel Approach to High Accuracy of Video-Based Microrheology Pei-Hsun Wu; Stephen H. Arce; Patrick R. Burney; and Yiider Tseng, University of Florida, Biophysical Journal, 96(1883), (2009)
Link To Paper

Materials & Methods:
Glycerol samples with suspended 100-nm carboxylated polystyrene fluorophores (Invitrogen Carlsbad CA) were made by well mixing at a 1:1000 volume ratio on a center area of a glass bottom dish (MatTek Ashland MA). Slides for tracking immobile particles were prepared by air drying 1:1000 volume ratio 100-nm carboxylated polystyrene fluorophores in ethanol onto a glass coverslip. The coverslip was mounted onto a glass slide with a drop of Fluoromount-G (SouthernBiotech Birmingham AL) and allowed to dry for 4 h before being sealed with nail polish.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Glycerol