Technical Reference #1876

1876.

Porous-wall hollow glass microspheres as novel potential nanocarriers for biomedical applications Shuyi Li; Lynsa Nguyen; Hairong Xiong; Meiyao Wang; Tom C.-C. Hu; Jin-Xiong She; Steven M. Serkiz; George G. Wicks; William S. Dynan, Medical College of Georgia, Nanomedicine: Nanotechnology; Biology; and Medicine, 6(1876), (2010)
Link To Paper

Keywords:
Drug delivery; Microsphere; Silica; Mesoporous; Controlled release; Single-chain antibody; Single-chain antibody variable fragment; Small interfering RNA

Materials & Methods:
Dry PW-HGMs (2–3 mg) were suspended in 50–100 μL of PBS containing 200 μg/mL of dextran 200 μg/mL protein or 2 μMnucleic acid and incubated at room temperature (20-25°C) for 5–10 minutes. An aliquot was transferred to a glass-bottom microwell dish (MatTek Corp. Ashland Massachusetts) for direct observation. The remainder was collected by gentle centrifugation washed with 0.5 mL of PBS or fetal bovine serum (FBS) and centrifuged again to remove excess dextran nucleic acid or protein. The pellet was resuspended in 50–100 μL of PBS or FBS for imaging. For sequential incubation experiments 2 μM DNA or siRNA was incubated with PWHGMs for 5–10 minutes FITC-dextran (70 kDa 200 μg/mL) was added and incubation was continued for another 5–10 minutes. Washing was performed as described. Microscopy was performed using a Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss MicroImaging Inc. Thornwood New York) with a 40× or a 63× oil objective or an Applied Precision Deltavision microscope (Applied Precision Inc. Issaquah Washington ) with a 20× or a 60× oil objective.

Microscopic Technique
laser scanning confocal microscopy

Cell Type(s)
PW-HGMs