Technical Reference #1875

1875.

Random Walk of Processive; Quantum Dot-Labeled Myosin Va Molecules within the Actin Cortex of COS-7 Cells Shane R. Nelson; M. Yusuf Ali; Kathleen M. Trybus; and David M. Warshaw, University of Vermont College of Medicine, Biophysical Journal, 97(1875), (2009)
Link To Paper

Materials & Methods:
COS-7 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) enriched with 10% (v/v) FBS with 5% CO2 at 37 C on 35 mm petri dishes with No. 1 coverglass bottoms (MatTek Ashland MA). These cells are extremely flat and tightly adherent to the coverglass which makes them good candidates for TIRF microscopy.

Microscopic Technique
TIRF Microscopy

Cell Type(s)
COS-& cells