Technical Reference #1866

1866.

Genetic engineered; biarsenical labelled influenza virus allows visualization of viral NS1 protein in living cells Yang Li; Xinya Lu; Junwei Li; Nathalie Bérubé; Kerri-Lane Giest; Qiang Liu; Deborah H. Anderson; and Yan Zhou, University of Saskatchewan, Journal of Virology, (2010)
Link To Paper

Materials & Methods:
For visualizing NS1 nuclear import A549 cells seeded in glass-bottom 35 mm 4 dishes (MatTek) were infected with PR8-411 at an MOI of 1. At 3 h.p.i. the media were 5 changed to Opti-MEM containing FlAsH (0.2 μM) EDT (50 μM) and β-6 mercaptoethanol (10 μM).

Microscopic Technique
Live-cell Fluorescence Microscopy

Cell Type(s)
A549 cells (human lung carcinoma cells)