Technical Reference #1864

1864.

Demyelination Increases Axonal Stationary Mitochondrial Size and the Speed of Axonal Mitochondrial Transport Sumiko Kiryu-Seo, Nobuhiko Ohno, Grahame J. Kidd, Hitoshi Komuro, and Bruce D. Trapp, Lerner Research Institute, Cleveland Clinic, The Journal of Neuroscience, 30(1864), (2010)
Link To Paper

Materials & Methods:
Before cell plating, poly-D-lysine-coated glass-bottom culture dishes (MatTek) were coated by Matrigel (BD Biosciences). A total of 5 104 cells was plated into the dishes. Five days after plating, 50 g/ml ascorbic acid was added to the medium to induce myelination; these cultures were maintained for 7–9 additional weeks. The medium was changed every 2–3 d.

Microscopic Technique
Electron Microscopy

Cell Type(s)
Rat DRG and Schwann cell mixed cultures