Technical Reference #185

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm tissue culture dishes containing a glass coverslip (MatTek Corp.; Ashland; MA)

185.

Visualization Of A Functional Gαq-Green Fluorescent Protein Fusion In Living Cells Hughes; T.; Zhang; H.; Logothetis; D.; Berlot; C., Yale University, The Journal of Biological Chemistry, 276(185), (2001)
Link To Paper

Abstract:
To investigate how G protein α subunit localization is regulated under basal and activated conditions we inserted green fluorescent protein (GFP) into an internal loop of Gαq. αq-GFP stimulates phospholipase C in response to activated receptors and inhib

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293