Technical Reference #1840

1840.

SAP102 Is a Highly Mobile MAGUK in Spines Chan-Ying Zheng; Ronald S. Petralia; Ya-Xian Wang; Bechara Kachar; and Robert J. Wenthold, National Institutes of Health - National Institute of Deafness and Other Communication Disorders, Journal of Neuroscience, 30(1840), (2010)
Link To Paper

Materials & Methods:
FRAP. Hippocampal neurons were cultured on poly-L-lysine-coated glass-bottom dishes (MatTek) at E18 and then transfected on 16–18 DIV. Neurons were used for FRAP experiment 4 d after transfection. The culture medium was exchanged with prewarmed Tyrode solution before experiments. Tyrode solution contained the following (in mM): 145 NaCl 5 KCl 10 HEPES 10 glucose 0.005 glycine 2.6 CaCl2 and 1.3 MgCl2 (pH adjusted to 7.4 with NaOH). Temperature was kept at 37°C using a Zeiss TempModule system. Depending on the experiments 10 M jasplakinolide (EMD) 100 M DL-APV and 20 M NBQX-disodium salt hydrate (Tocris Bioscience) were added into Tyrode solution 30–60 min before FRAP recording and during FRAP recording. Control coverslips were incubated with prewarmed Tyrode solution or 1% DMSO instead of the drugs.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Hippocampal neurons