Technical Reference #1837

1837.

CENP-A Reduction Induces a p53-Dependent Cellular Senescence Response To Protect Cells from Executing Defective Mitoses Kayoko Maehara; Kohta Takahashi; and Shigeaki Saitoh, Kurume University, Molecular and Cellular Biology, 30(1837), (2010)
Link To Paper

Materials & Methods:
Live cell analysis. TIG3 cells expressing H2B-EGFP were cultured on 35-mm glass-bottom dishes (MatTek) in DMEM and observed using a Leica ASMDW live cell imaging system (Leica Microsystems) equipped with a 63 objective lens (NA 1.30) and a temperature control unit. The cells were maintained at 37°C under a humidified atmosphere of 5% CO2. Three-dimensional stacks of Zsectioned images (2- m interval) were collected using a 30-ms exposure every 4 min. For deconvolution the nonblind algorithm of ASMDW software was applied.

Microscopic Technique
Live Cell Imaging

Cell Type(s)
TIG3 cells