Technical Reference #1830

1830.

Burkholderia cenocepacia Creates an Intramacrophage Replication Niche in Zebrafish Embryos; Followed by Bacterial Dissemination and Establishment of Systemic Infection Annette C. Vergunst; Annemarie H. Meijer; Stephen A. Renshaw; and David O'Callaghan, INSERM ESPRI 26, Infection and Immunity, 78(1830), (2010)
Link To Paper

Materials & Methods:
Microscopic analysis. A Leica DM IRB inverted microscope equipped for bright-field differential interference contrast (DIC) and fluorescence imaging was used. GFP and DSRed were excited using a 100-W mercury lamp and fluorescence was detected using filter sets L5 (band pass [BP] 480/40; beam splitter [BS] 505; emission BP527/30) and N2.1 (515 to 560; BS 580; emission long pass [LP] 590) respectively. For imaging we used a Coolsnap fx (Roper Scientifique) and MetaVue software and images were processed further using Adobe Photoshop. Embryos were transferred to E3 containing MS222 in glassbottom dishes (MatTek Corp. Ashland MA) for direct visualization using 40 63 and 100 oil immersion objectives.

Microscopic Technique
Inverted and Fluorescence Microscopy

Cell Type(s)
Zebrafish embryos