Technical Reference #1827

1827.

Src Induces Podoplanin Expression to Promote Cell Migration* Yongquan Shen; Chen-Shan Chen; Hitoshi Ichikawa; and Gary S. Goldberg, University of Medicine and Dentistry of New Jersey, Journal of Biological Chemistry, 285(1827), (2010)
Link To Paper

Keywords:
Cancer; Cell; Cell/Cell-cell interaction; Phosphorylation/Kinases/Tyrosine; Cell junctions; Cell migration

Materials & Methods:
Immunofluorescence Microscopy—Cells (300000/dish) were cultured on 35-mm poly-D-lysine-coated glass-bottomed culture dishes (MatTek P35GC-1.5-14-C) for 24 h. Cells were fixed with 2% paraformaldehyde permeabilized with 0.2% Triton X-100 in phosphate-buffered saline for 10 min washed three times with 0.1% Tween 20 in phosphate-buffered saline followed by 1% bovine serum albumin in phosphate-buffered saline for 30 min incubated with Pdpn antiserum (1:100) overnight at 4 °C washed and then labeled with goat anti-Syrian hamster IgG conjugated to Alexa Fluor 488 (Molecular Probes A21110). Cells were visualized on Zeiss Axiovert or PASCAL microscope systems as described (14 15).

Microscopic Technique
Immunofluorescence Microscopy

Cell Type(s)
Embryonic fibroblasts; Cx43Ko; CasKo cells