Technical Reference #1823

1823.

Virological Synapse-Mediated Spread of Human Immunodeficiency Virus Type 1 between T Cells Is Sensitive to Entry Inhibition Nicola Martin; Sonja Welsch; Clare Jolly; John A. G. Briggs; David Vaux; and Quentin J. Sattentau, University of Oxford - The Sir William Dunn School of Pathology, Journal of Virology, 84(1823), (2010)
Link To Paper

Materials & Methods:
Life span of HIV-1 R5 T-cell VS. To investigate the possibility that inhibitors might interfere with HIV-1 spread across existing VS we first established the longevity of R5 VS in T cells. For this we assumed that long-lived conjugates formed between HIV-1-infected and uninfected receptor-expressing CD4 T cells represented VS a proportion of which would be functional in cell-cell transfer of HIV-1 infection. Differentially labeled JktBaL cells or control uninfected Jurkat cells were mixed with uninfected primary CD4 T cells and their interactions were imaged using time-lapse LSCM. A frame from a representative movie (see Movie S1 in the supplemental material) is shown in Fig. 3A in which multiple conjugates between infected (red) and uninfected target cells (green) are identified by arrows.

Microscopic Technique
Time-lapse LSCM

Cell Type(s)
JktBaL cells and CD4z+T cells