Technical Reference #1800
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
placed
apical face down in a dish with a coverslip bottom (no. 1.5; MatTek;
Ashland; MA) 
1800. |
PTH-induced internalization of apical membrane NaPi2a: role of actin and myosin VI
Judith Blaine; Kayo Okamura; Hector Giral; Sophia Breusegem; Yupanqui Caldas; Andrew Millard; Nicholas Barry; Moshe Levi,
University of Colorado Denver,
American Journal of Physiology - Cell Physiology,
297(1800),
(2009)
Link To Paper
Keywords:
apical total internal reflection fluorescence microscopy; parathyroid hormone Materials & Methods:
Opossum kidney proximal tubule cells that are responsive to PTH (OKP cells) were a gift from Dr. J. Cole (Univ. of Memphis Memphis TN) and were grown in DMEM-F-12 (Invitrogen Carlsbad CA) supplemented with 10% FBS 50 U/ml penicillin and 50 g/ml streptomycin. For experimental work cells were seeded on porous membrane inserts (Corning Lowell MA). At 90% confluency cells were transfected overnight using Lipofectamine 2000 (Invitrogen) according to the instructions provided by the manufacturer. The next day the cells were placed in DMEM-F-12 supplemented with 0.2% FBS to synchronize the cells. Microscopic Technique
Fluorescence Microscopy Cell Type(s)
OKP cells |
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