Technical Reference #1796

1796.

A Reporter of Local Dendritic Translocation Shows Plaque-Related Loss of Neural System Function in APP-Transgenic Mice Melanie Meyer-Luehmann; Matthew Mielke; Tara L. Spires-Jones; Will Stoothoff; Phill Jones; Brian J. Bacskai; Bradley T. Hyman, Harvard Medical School, Journal of Neuroscience, 29(1796), (2009)
Link To Paper

Materials & Methods:
Cell culture. Primary neuronal cell cultures were prepared from mixed cortical-hippocampal neurons generated from CD1 mice at embryonic day 15–16. Cells were plated on poly-D-lysine-coated coverslipped 35mmdishes (MatTek Cultureware) in neurobasal media (Invitrogen) containing 10% FBS. The neurons were maintained and allowed to mature in neurobasal media containing 2% B27 supplement (Invitrogen) for 6–12 d before use.

Cell Type(s)
Neurons - cortical-hippocampal