Technical Reference #1785

1785.

Heterodimerization of ORL1 and Opioid Receptors and Its Consequences for N-type Calcium Channel Regulation Rhian M. Evans; Haitao You; Shahid Hameed; Christophe Altier; Alexandre Mezghrani; Emmanuel Bourinet; Gerald W. Zamponi, University of Calgary, Journal of Biological Chemistry, 285(1785), (2010)
Link To Paper

Keywords:
N-type channel; Opioid receptors; Heterodimers; Nociceptin; DAMGO; Internalization; G Protein

Materials & Methods:
Cell Culture and Transfection—tsA-201 cells were cultured and transfected as previously reported (14). Cells were replated at 8% confluence on either poly-L-lysine-coated glass coverslips or 35-mm glass-bottomed dishes (MatTek Corp.). Cells used for electrophysiology experiments were moved to 30 °C after transfection whereas those used for confocal microscopy or Western blotting were maintained at 37 °C until used. In cells used for electrophysiological recordings enhanced yellow fluorescent protein (YFP2 0.5 g; Clontech) was included as a transfection marker.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
tsA-201 cells