Technical Reference #1772

1772.

Cannabinoid 1 Receptor and Interleukin-6 Receptor Together Induce Integration of Protein Kinase and Transcription Factor Signaling to Trigger Neurite Outgrowth Yana Zorina; Ravi Iyengar; Kenneth D. Bromberg, Mount Sinai School of Medicine, Journal of Biological Chemistry, 285(1772), (2010)
Link To Paper

Materials & Methods:
Materials and Antibodies—HU-210 was obtained from Sigma and mouse IL-6 was from Invitrogen. PP2 PD98059 and LY294002 were from Calbiochem. Polyclonal rabbit antibodies directed against phospho-STAT3 total STAT3 phospho-SHP2 total SHP2 MAP2 phospho-MAPK1/2 total MAPK1/2 phospho-Erk5 total Erk5 phospho-Akt total Akt and monoclonal mouse antibodies directed against Tau were from Cell Signaling Technology Inc. Monoclonal mouse antibodies against -tubulin were from Sigma.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
N2A cells; PC12 cells