Technical Reference #1772

1772.

Cannabinoid 1 Receptor and Interleukin-6 Receptor Together Induce Integration of Protein Kinase and Transcription Factor Signaling to Trigger Neurite Outgrowth Yana Zorina, Ravi Iyengar, Kenneth D. Bromberg, Mount Sinai School of Medicine, Journal of Biological Chemistry, 285(1772), (2010)
Link To Paper

Materials & Methods:
Materials and Antibodies—HU-210 was obtained from Sigma, and mouse IL-6 was from Invitrogen. PP2, PD98059, and LY294002 were from Calbiochem. Polyclonal rabbit antibodies directed against phospho-STAT3, total STAT3, phospho-SHP2, total SHP2, MAP2, phospho-MAPK1/2, total MAPK1/2, phospho-Erk5, total Erk5, phospho-Akt, total Akt, and monoclonal mouse antibodies directed against Tau were from Cell Signaling Technology Inc. Monoclonal mouse antibodies against -tubulin were from Sigma.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
N2A cells; PC12 cells