Technical Reference #1767

1767.

Receptor-associated Protein Interacts with Amyloid-B Peptide and Promotes Its Cellular Uptake Takahisa Kanekiyo; Guojun Bu, Washington University School of Medicine Xiamen University, Journal of Biological Chemistry, 284(1767), (2009)
Link To Paper

Materials & Methods:
Materials—A 40 A 42 and 5(6)-carboxyfluorescein (FAM)-labeled A 40 were purchased from AnaSpec (San Jose CA). A peptides were dissolved in dimethyl sulfoxide at 200 M and kept in 80 °C before use. Recombinant RAP and Mesd (mesoderm development) were produced and purified as described previously (27 28).

Microscopic Technique
confocal laser scanning fluorescence microscopy

Cell Type(s)
HBVSMC; MEF1 cells; MEF2 cells; U87 & SH-SY5Y cell