Technical Reference #1760

1760.

Plasma Membrane Nucleolin Is a Receptor for the Anticancer Aptamer AS1411 in MV4-11 Leukemia Cells Sridharan Soundararajan; Li Wang; Vijayalakshmi Sridharan; Weiwei Chen; Nigel Courtenay-Luck; David Jones; Eleanor K. Spicer; Daniel J. Fernandes, Medical University of South Carolina, Molecular Pharmacology, 76(1760), (2009)
Link To Paper

Materials & Methods:
All antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz CA). PCR primers were synthesized by Operon Technologies (Alameda CA). Immobilon-P polyvinylidene difluoride transfer membranes and all other chemicals and supplies were purchased from Thermo Fisher Scientific (Waltham MA). AS1411 having the sequence 5 -d(GGTGGTGGTGGTTGTGGTGGTGGTGG)- 3 and 5 -FITC-AS1411 were obtained from Antisoma Research Ltd. (London UK). CRO-26 (Integrated DNA Technologies Coralville IA) is a cytosine-rich oligonucleotide in which each dG of AS1411 is replaced with dC. The oligonucleotides were dissolved in serum-free tissue culture medium before addition to cell cultures.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MV4-11 cells; K-562 leukemia cells