Technical Reference #1759

1759.

Visualizing sodium dynamics in isolated cardiomyocytes using fluorescent nanosensors J. Matthew Dubach; Saumya Das; Anthony Rosenzweig; Heather A. Clark, The Charles Stark Draper Laboratory, PNAS, 106(1759), (2009)
Link To Paper

Keywords:
Cellular imaging; ion channels; sodium sparks

Materials & Methods:
Human embryonic kidney cells stably expressing the sodium channel hNaV1.7 (HEK PN1) were obtained from Merck. Veratridine was brought upinDMSOto 50 or 20mMand stored at 20 °C for less than 3 months. Astock solution of TTX was made in distilled water to 1 mM or 10 M and stored at 20 °C. Gramicidin was brought up in DMSO to 5 mM and stored at 4 °C. CoroNa green was obtained from Invitrogen and a stock solution was made into 1-mM aliquots in DMSO and stored at 20 °C before use. All chemicals were purchased from Sigma unless otherwise noted.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK PN1 cells