Technical Reference #1754

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
CHO-K1 and CHO Flp-In cells were seeded in 35-mm dishes with glass bottoms (MatTek Corp.; Ashland; MA)

1754.

Activation of Hormone-sensitive Lipase Requires Two Steps; Protein Phosphorylation and Binding to the PAT-1 Domain of Lipid Droplet Coat Proteins Hong Wang; Liping Hu; Knut Dalen; Heidi Dorward; Amy Marinkiewicz; Deanna Russell; Dawei Gong; Constantine Londos; Tomohiro Yamaguchi; Cecilia Holm; Mark A. Rizzo; Dawn Brasaemle; Carole Sztalryd, University of Maryland School of Medicine National Institutes of Health The State University of New Jersey Lund University University of Hyogo, Journal of Biological Chemistry, 284(1754), (2009)
Link To Paper

Materials & Methods:
Rabbit anti-GFP antibody was from Clontech and mouse anti-GFP antibody was from Covance (Princeton NJ). Antibodies raised in rabbits against HSL perilipin ADFP LSDP5 and CGI-58 were previously characterized (12 21 22). Rabbit anti ATGL antibody was from Cell Signaling Technology (Danvers MA). Horseradish peroxidase-conjugated goat anti-rabbit IgG and donkey anti-mouse IgG were purchased from Santa Cruz Biotechnology (Santa Cruz CA). Rabbit serum was from Rockland Immunochemicals (Gilbertsville PA). Mouse anti-rabbit IgG was from Jackson ImmunoResearch (West Grove PA). CHO-K1 and CHO Flp-In cells were obtained from ATCC (Manassas VA) and Invitrogen respectively.

Microscopic Technique
Fluorescence and confocal microscopy

Cell Type(s)
CHO-K1 and CHO Flp-In cells