Technical Reference #1750

1750.

Regulation of the Proteasome by Neuronal Activity and Calcium/Calmodulin-dependent Protein Kinase II Stevan N. Djakovic; Lindsay A. Schwarz; Barbara Barylko; George N. DeMartino; Gentry N. Patrick, University of California San Diego, Journal of Biological Chemistry, 284(1750), (2009)
Link To Paper

Materials & Methods:
Antibodies and Reagents— 2 proteasome (MCP21 monoclonal antibody (mAb)) core proteasome (polyclonal antibody) and Rpt6 (mAb) antibodies were purchased from Biomol. GFP antibody (full-length polyclonal antibody) was from Santa Cruz Biotechnology. The following pharmacological reagents were used. 2-Amino-5-phosphonopentanoic acid (APV) BIC nimodipine TTX and 6-cyano-7-nitroquinoxaline-23-dione (CNQX) were from Tocris Bioscience. MG132 was from Peptide Institute. Cycloheximide was from MP Biomedicals. Adamantane-acetyl-(6-aminohexanoyl)(3)-(leucinyl)(3)-vinyl-(methyl)-sulfone (vinyl sulfone) epoxomicin ( )- -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) KN-93 and myristoylated autocamtide-2-related inhibitory peptide (AIP) were from Biomol. Calpeptin was from EMD Biosciences. Suc-LLVY-AMC fluorogenic substrate was from Biomol. Purified calmodulin (human recombinant) and CaMKII (full-length human recombinant) were from Biomol.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Neuron - Hippocampal