Technical Reference #1747

1747.

Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation Yuji Chikashige; Miho Yamane; Kasumi Okamasa; Chihiro Tsutsumi; Tomoko Kojidani; Mamiko Sato; Tokuko Haraguchi; Yasushi Hiraoka, National Institute of Information and Communications Technology, Journal of Cell Biology, 187(1747), (2009)
Link To Paper

Materials & Methods:
Strains culture media and induction of meiosis The genotypes of the strains used in this paper are shown in Table I. The swi6 and taz1 strains were gifts from J. Kanoh (Osaka University Suita Osaka Japan) and the TAS-ura4+-tel2(L) strain was a gift from R.C. Allshire (Medical Research Council Edinburgh Scotland UK).YE YES or EMM2 were used for routine mitotic culture of S. pombe cells. ME agar plates were used for the induction of meiosis and spore formation. S. pombe h90 cells grown in a liquid medium were transferred to an ME plate at 26°C to induce meiosis. Chemical compositions of YE YES EMM2 and ME media are described in Moreno et al. (1991).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
S. pombe cells