Technical Reference #1745

1745.

Differential Cytokine Production and Bystander Activation of Autoreactive B Cells in Response to CpG-A and CpG-B Oligonucleotides Ana M. Avalos; Eicke Latz; Betty Mousseau; Sean R. Christensen; Mark J. Shlomchik; Frances Lund; Ann Marshak-Rothstein, Boston University School of Medicine, Journal of Immunology, 183(1745), (2009)
Link To Paper

Materials & Methods:
AM14 transgenic mice and AM14 TLR9 / mice have been described (14 16). AM14 knock-in mice were generated by insertion of the AM14 H chain into the endogenous H chain locus (Christensen and Shlomchik manuscript in preparation) and the VK8 L chain into the endogenous K chain locus (provided by Dr. M. Weigert) and were maintained at the Laboratory Animal Sciences Center at Boston University School of Medicine. All animal procedures were performed under the guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care and approved by Boston University School of Medicine Institutional Animal Care and Use Committee.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Mouse splenic B cells