Technical Reference #1737

1737.

Prokineticin 2 Modulates the Excitability of Area Postrema Neurons in vitro in the Rat Matthew V. Ingves; Alastair V. Ferguson, Queen's University, American Journal of Physiology - Regulatory; Integrative and Comparative Phsyiology, 298(1737), (2010)
Link To Paper

Keywords:
circadian regulation; circumventricular organ; enkephalin; sodium channels

Materials & Methods:
Dissociated cells were plated on 35mm uncoated glass bottom culture dishes (MatTek Ashland MA) at a low density (~10 cells/mm2) to ensure synaptic contacts did not form and incubated at 37°C in 5% CO2. Electrophysiological experiments were performed on neurons maintained in culture for 1-5 days during which time some small process were developed none of which were seen (visual inspection using patch clamp microscope) to make contact with other cells.

Microscopic Technique
Light Microscopy

Cell Type(s)
Area postrema (AP) tissue cells