Technical Reference #1731

1731.

Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors Jinlan Chang; Fred D. Mast; Andrei Fagarasanu; Dorian A. Rachubinski; Gary A. Eitzen; Joel B. Dacks; Richard A. Rachubinski, University of Alberta, Journal of Cell Biology, 187(1731), (2009)
Link To Paper

Materials & Methods:
Strains and culture conditions The Y. lipolytica strains used in this study are listed in Table S1. Strains were cultured at 30°C unless otherwise indicated. Strains containing plasmid pUB4 were cultured in YPD or YPBO supplemented with hygromycin B at 125 μg/ml. Strains containing plasmid pTC3 were cultured in YNA or YNO medium supplemented with lysine at 50 μg/ml. Media components were as follows: YPD 1% yeast extract 2% peptone and 2% glucose; YPBO 0.3% yeast extract 0.5% peptone 1% Brij 35 1% [vol/vol] oleic acid 0.5% K2HPO4 and 0.5% KH2PO4; YNO 1.34% yeast nitrogen base without amino acids 0.05% [wt/vol] Tween 40 0.2% [wt/vol] oleic acid; and YNA 1.34% yeast nitrogen base without amino acids and 2% sodium acetate.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Y. lipolytica strains