Technical Reference #1723

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
The cells were cultivated in glass-bottom culture dishes p35 (MatTek; Ashland; MA; part no. P35GCol-0–14-C; collagen coated)

1723.

Oral PEG 15-20 protects the intestine against radiation: role of lipid rafts Vesta Valuckaite; Olga Zaborina; Jason Long; Martin Hauer-Jensen; Junru Wang; Christopher Holbrook; Alexander Zaborin; Kenneth Drabik; Mukta Katdare; Helena Mauceri; Ralph Weichselbaum; Millicent A. Firestone; Ka Yee Lee; Eugene B. Chang; Jeffrey Matthews, Bioengineering Institute for Advanced Surgery and Endoscopy University of Chicago Argonne National Laboratory University of Arkansas for Medical Sciences and Surgical Service, American Journal of Physiology - Gastrointestinal and Liver Physiology, 297(1723), (2009)
Link To Paper

Keywords:
Pseudomonas aeruginosa; gut-derived sepsis; apoptosis; radiation injury

Materials & Methods:
P. aeruginosa strains PAO1 and its derivative strain PAO1/enhanced green fluorescent protein (EGFP) harboring the egfp gene encoding green fluorescent protein EGFP on the pUCP24 plasmid vector expressed under Plac promoter (52) were used. Rat IECs (IEC-18 cells passages 30-34) were used in all experiments. IEC-18 cells were routinely cultured in plastic culture flasks containing DMEM media (DMEM high glucose 4.5 g/l containing 5% heat-inactivated fetal bovine serum and 0.1 U/ml insulin).

Microscopic Technique
Atomic Force Microscopy, Fluorescent Confocal Microscopy

Cell Type(s)
PAO1; IEC-18