Technical Reference #1721

1721.

α3β1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development Ralf S. Schmid; Stephanie Shelton; Amelia Stanco; Yukako Yokota; Jordan A. Kreidberg and E. S. Anton, The University of North Carolina School of Medicine, Development, 131(1721), (2004)
Link To Paper

Abstract:
We show that α3 integrin mutation disrupts distinct aspects of neuronal migration and placement in the cerebral cortex. The preplate develops normally in α3 integrin mutant mice. However time lapse imaging of migrating neurons in embryonic cortical slices indicates retarded radial and tangential migration of neurons but not ventricular zone-directed migration.

Keywords:
Cerebral cortex; Migration; Adhesion

Materials & Methods:
Generation and characterization of targeted mutation in mouse α3 integrins was described by Kreidberg et al. (Kreidberg et al. 1996). Genotypes of the embryos used were determined by PCR as described earlier (DiPersio et al. 1997). Thy1-GFP transgenic lines expressing GFP in layer 6 (line I; a gift from Drs G. Feng and J. R. Sanes) were phenotyped as described by Feng et al. (Feng et al. 2000).

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Neurons