Technical Reference #1659

1659.

Bovine Lactadherin as a Calcium-Independent Imaging Agent of Phosphatidylserine Expressed on the Surface of Apoptotic HeLa Cells Lasse N Waehrens; Christian W Heegaard; Gary E Gilbert; Jan T Rasmussen, University of Aarhus, Journal of Histochemistry and Cytochemistry, 57(1659), (2009)
Link To Paper

Abstract:
Bovine lactadherin holds a stereo-specific affinity for phosphatidyl-L-serine (PS) membrane domains and binds at PS concentrations lower than the benchmark PS probe annexin V. Accordingly lactadherin has recognized PS exposure on pre-apoptotic immortali

Keywords:
Lactadherin; annexin V; HeLa; phosphatidyserine; confocal microscopy; MFG-E8.

Materials & Methods:
HeLa cells were maintained in DMEM +GlutaMax ® with 10% fetal bovine serum and 1% Pen/Strep at 37°C in 5% CO2. For microscopy cells were grown on 35 mm petri dish 14 mm Microwell No. 1.5 coverglass (0.16-0.19 mm) from the MatTek Corp MA USA

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa