Technical Reference #1626

1626.

Pannexin1 in the Outer Retina of the Zebrafish; Danio Rerio N. PROCHNOW;a1 S. HOFFMANN;a;c1 R. VROMAN;e1 J. KLOOSTER;e S. BUNSE;a;d M. KAMERMANS;e;f R. DERMIETZELa AND G. ZOIDLa;b*, Ruhr University, Neuroscience, 162(1626), (2009)

Abstract:
of Amsterdam The NetherlandsAbstract—In the retina chemical and electrical synapsescouple neurons into functional networks. New candidatesencoding for electrical synapse proteins have recently emerged. Inthe present study we determined the loca

Keywords:
pannexin; retina; electrical synapse; horizontal cell

Materials & Methods:
Cell culture and transfection N2a cells were maintained at 37 °C and 5% CO2 in D-MEM containing 4500 mg/l D-glucose and L-glutamine (Invitrogen) supplemented with 5% fetal calf serum (Perbio Science Deutschland Bonn Germany) 1% L-glutamine (PAA Laboratories GmbH Pasching Austria) 1% penicillin/streptomycin (PAA) 1% nonessential amino acids (aa) (PAA) and 1% sodium pyruvate (PAA). Transient transfections were carried out using the Effectene®4 Transfection Reagent Kit (Qiagen GmbH Hilden Germany). For localization studies 9000 cells and for dye uptake assays 15000 cells were seeded on glass bottom culture dishes (MatTek Corporation Ashland MA USA). Plasmids (300 ng) were transfected and cells studied after 48 h.

Microscopic Technique
Electron Microscopy, Immunoelectron Microscopy

Cell Type(s)
N2A