Technical Reference #1615

1615.

Increased Beta Amyloid Levels in the Choroid Plexus Following Lead Exposures and the Involvement of Low Density Lipoprotein Receptor Protein-1 Mamta Behl; Yanshu Zhang; Andrew Monnot; Wendy Jiang; Wei Zheng, Purdue University, Toxicology and Applied Pharmacology, 240(1615), (2009)
Link To Paper

Abstract:
The choroid plexus a barrier between the blood and cerebrospinal fluid (CSF) is known toaccumulate lead (Pb) and also possibly function to maintain brain’s homeostasis of Aβ animportant peptide in the etiology of Alzheimer’s disease. This study was

Keywords:
blood–CSF barrier; Pb; Aβ; LRP1; choroid plexus; blood-brain barrier; Alzheimer’s disease

Materials & Methods:
The effects of Pb exposure on Aβ uptake in choroidal epithelial cells were qualitatively assessed using confocal microscopy. Z310 cells were plated on 35-mm glass plates (MatTek Ashland MA) and grown until they reached about 85% confluence (2–3 days). The cells were treated with 10 μM Pb as Pb acetate for 24 h in DMEM medium. Following exposure the cells were washed 3 times with phosphate buffered saline (PBS) to remove all remnants of Pb in the dish. Cells were then incubated with 200 mL of 2 μM fluorescent labeled Aβ for one hour followed by three PBS washes. Fresh cell culture medium was then added and the preparations were observed under a confocal microscope.

Microscopic Technique
Confocal Microscopy, Confocal Immunofluorescence Microscopy

Cell Type(s)
Z310