Technical Reference #1598

1598.

Evidence For A Second; High Affinity Gbr Binding Site On Gai1 (Gdp) Subunits Wang; J.; Sengupta; P.; Guo; Y.; Golebiewska; U.; Scarlata; S., Stony Brook University, The Journal of Biological Chemistry, 284(1598), (2009)
Link To Paper

Abstract:
It is well known that Gi1(GDP) binds strongly to G subunits to form the Gi1(GDP)-G heterotrimer and thatactivation to Gi1(GTP) results in conformational changesthat reduces its affinity for G subunits. Previous studies ofG protein subunit interac

Materials & Methods:
His6-Gi1 proteins were expressed in Escherichia coli and purified as previously described.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
HEK-293