Technical Reference #1597

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
3.5 cm glass bottom dishes (MatTek; Ashland; MA); glass bottom 96 well plates (MatTek)

1597.

Specificity Of Cytoplasmic Dynein Subunits In Discrete Membrane-Trafficking Steps Palmer; K.;Hughes; H.; Stephens; D., University of Bristol, Molecular Biology of the cell, 20(1597), (2009)
Link To Paper

Abstract:
The cytoplasmic dynein motor complex is known to exist in multiple forms but few specific functionshave been assigned to individual subunits. A key limitation in the analysis of dynein in intactmammalian cells has been the reliance on gross perturbati

Keywords:
dynein; golgi; membrane traffic

Materials & Methods:
HeLa (ATTC CRL-2) and HeLa cells stably expressing GRASP65-GFP (Lane et al. 2002) were maintained in DMEM supplemented with 10% fetal calf serum. For imaging cells were grown on 3.5

Microscopic Technique
Wide-field Microscopy

Cell Type(s)
HeLa