Technical Reference #1595

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom culture dishes (MatTek Corporation; Ashland; MA; USA)

1595.

Linker Histone H1 Is Present In Centromeric Chromatin Of Living Human Cells Next To Inner Kinetochore Proteins Orthaus; S.; Kelement; K.; Happel; N.; Hoischen; C.; Diekmann; S., Fritz Lipmann Institute, Nucleic Acid Research, 37(1595), (2009)
Link To Paper

Abstract:
The vertebrate kinetochore complex assemblesat the centromere on a-satellite DNA. In humansa-satellite DNA has a repeat length of 171 bp slightlylonger than the DNA in the chromatosome containingthe linker histone H1. The centromere-bindingprot

Materials & Methods:
For indirect immunofluorescence detection HeLa cells (from the DSMZ Braunschweig Germany) were fixed with 3% paraformaldehyde (pFA) in phosphate buffered

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa