Technical Reference #1593

1593.

Gpi- Anchored Human Placental Alkaline Phosphatase Has A Nonpolarized Distribution On The Cell Surface Of Mouse Cerebellar Granule Neurons In Vitro Katherine M. Kollins; Sharon K. Powell; Rodolfo J. Rivas, University of London, Journal of Neurobiology, 39(1593), (1999)
Link To Paper

Abstract:
The glycosyl phosphatidylinositol(GPI) lipid anchor which directs GPI-anchored proteinsto the apical cell surface in certain polarized epithelialcell types has been proposed to act as an axonalprotein targeting signal in neurons. However as sev

Keywords:
glycosyl phosphatidylinositol; axons; dendrites; neuronal polarity; cerebellar granule neuron

Materials & Methods:
The replication-incompetent MFG retroviral vector which uses the murine Moloney virus LTR as a promoter to direct exogenous gene expression (Pear et al. 1993)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK