Technical Reference #1590

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
polylysine coated Mattek dishes

1590.

Recombinant Mitochondrial Transcription Factor A With N-Terminal Mitochondrial Transduction Domain Increases Respiration And Mitochondiral Gene Expression Shilpa Iyer; Ravindar R. Thomas; Francisco R. Portell; Lisa D. Dunham; Caitlin K. Quigley; James P. Bennett Jr, University of Virginia, Mitochondrion, 9(1590), (2009)
Link To Paper

Abstract:
We developed a scalable procedure to produce human mitochondrial transcription factor A (TFAM) modifiedwith an N-terminal protein transduction domain (PTD) and mitochondrial localization signal (MLS)that allow it to cross membranes and enter mitochond

Keywords:
TFAM; respiration; mitochondrial gene expression; respiratory proteins

Materials & Methods:
We included a HA epitope after the 11-Arginine PTD based on reports that the HA epitope facilitates cytosolic escape of transduced protein after macropinocytosis

Microscopic Technique
Confocal Microscopy

Cell Type(s)
DS9 Cybrid cells containing the G11778A LHON mutat