Technical Reference #1580

1580.

Pml# Orchestrates The Nulcear Dynamics And Function Of Tip60 Quan Wu, Haixiang Hu, Jianping Lan, Chibuzo Emenari, Zhiyong Wang, Kang-Sang Chang, He Huang, Xuebiao Yao, Zhejiang University School of Medicine, MD Anderson Cancer Center, The Journal of Biological Chemistry, 284(1580), 8751, (2009)
Link To Paper

Abstract:
The promyelocytic leukemia (PML) protein is a major componentto govern the PML nuclear body (NB) assembly and function.Although it is well defined that PML NB is a site recruitingsumoylated proteins, the mechanism by whichPMLprotein regulatesthe p

Materials & Methods:
Cell Culture and Transfection and Small Interfering RNA Experiment—HeLa cells, H1299 cells, U2OS cells, and 293T cells were obtained fromATCC(Manassas, VA) and cultured in media

Microscopic Technique
Inverted Microscopy

Cell Type(s)
HeLa, U205, H1299