Technical Reference #1577

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass-bottom dishes were obtained from MatTek (Ashland; MA)

1577.

Digital Fluorescence Analysis Of Trafficking Of Single Endosomes Containing Low-Density Lipoprotein In Adrenocortical Cells: Facilitation Of Centripetal Motion By Adrenocorticotropic Hormone Tetsuya Kimoto; Makoto Yamada; Tomomitsu Ichikawa; Daisaku Honma; Richard J. Cherry; Ian E.G. Morrison; Suguru Kawato, University of Tokyo, Molecular and Cellular Endocrinology, 307(1577), (2009)

Abstract:
Imaging of trafficking of endosomes containing low-density lipoprotein (LDL) is useful to analyze cholesteroltransport in adrenocortical cells. At 60 min after the application of fluorescently labeled LDL toadrenocortical cells individual endosomes c

Keywords:
fluorescence imaging; LDL; adrenocotical cells; endosomes; single particle tracking; ACTH

Materials & Methods:
Dioctadecyl tetramethylindocarbocyanine perchlorate (DiI)was purchased from Molecular Probes (Eugene OR)ACTH1-24 peptide

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
adrenocortical