Technical Reference #1566

1566.

Activation Of Pho And Pho-Associated Kinase By Gpr54 And Kiss1 Metastasis Supressor Gene Product Induces Changes Of Cell Morphology And Contributes To Apoptosis JM Navenot; N Fujii; SC Peoper, Medical College of Georgia, Molecular Pharmacology, 76(1566), (2009)
Link To Paper

Abstract:
The mechanism of action of the metastasis suppressor KiSS1and its receptor GPR54 is still incompletely characterized. Althoughthe loss of KiSS1 expression by tumor cells has beenassociated with a metastatic phenotype the nature of the cellulartar

Materials & Methods:
The human cell line HEK-293T was modified to express GPR54 with an N-terminal Myc epitope tag by transfection with a pcDNA3.1 construct

Microscopic Technique
Fluorescence Microscopy, Live Cell

Cell Type(s)
HEK-293, lymphoid cells