Technical Reference #1546

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom 35 mm dishes (Mat-Tek; Ashland; MA)

1546.

Kv2.1 Potassium Channels Are Retained Within Dynamic Cell Surface Microdomains That Are Defined By A Perimeter Fence Kristen M.S. O'Connell; Annah S. Rolig; jennifer D. Whitesell; Michael M. Tanmkun, Colorodo State University, The The Journal of Neuroscience, 26(1546), (2006)
Link To Paper

Abstract:
Ion channel localization to specific cell surface regions is essential for proper neuronal function.

Keywords:
Kv channels; restricted diffusion; membrane insertion; quantum dot tracking; fluorescence microscopy; hippocampal neurons

Materials & Methods:
Vectors containing green fluorescent protein (GFP)–Kv2.1–hemagglutinin (HA) (O'Connell and Tamkun 2005) or GFP-Kv2.1-loopBAD were electroporated into HEK 293 cells (American Type Culture Collection Manassas VA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293