Technical Reference #1539

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass-bottom microwell dishes for confocal studies were obtained from Mat-Tek

1539.

Differential Impact Of Intracellular Carboxyl Terminal Domains On Lipid Raft Localization On The Murine Gonadotropin-Releasing Hormone Receptor Amy Navratil; Todd Farmerie; jan Bogerd; Terry Nett; Colin Clay, Utrecht University, Biology of Reproduction, 74(1539), (2006)
Link To Paper

Abstract:
The mammalian type I GNRH receptor (GNRHR) is uniqueamong G protein-coupled receptors (GPCRs) because of theabsence of an intracellular C-terminus. Previously we havefound that the murine GNRHR is constitutively localized to lowdensitymembrane mi

Keywords:
gonadotropin-releasing hormone receptor; mechanisms of hormone action; pituitary

Materials & Methods:
Antihemagglutinin (anti-HA) antibody was purchased from Roche. Secondary antibodies as well as those against phosphorylated mitogenactivated protein kinase (phospho-MAPK; sold as ‘‘p-ERK (E-4)") and MAPK3

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CHO