Technical Reference #1536

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass bottom culture dishes were purchased from Mat Tek (USA)

1536.

Enzyme-Linked Sensitive Fluorometric Imaging Of Glutamate Release From Cerebral Neurons Of Chick Embryos Yoshinori Akagi; Atsuko Hashigasako; Patrick Degenaar; Shinichirou Iwabuchi; Quamrul Hasan; Yasutaka Morita; Eiichi Tamiya, Japan Advanced Institute of Science and Technology, The The Journal of Biological Chemistry, 134(1536), (2003)
Link To Paper

Abstract:
This paper describes a method for imaging the endogenous release of glutamate fromcerebral neurons. This method is based on the reactions of glutamate oxidase andperoxidase and on the detection of hydrogen peroxide by a fluorescent substrate ofpero

Keywords:
bioimaging; confocal microscope; enzyme sensor; fluorescence; glutamate; neuronal network

Materials & Methods:
Materials—Glutamate oxidase was purchased from Seikagaku (Japan). Peroxidase and glutamate were purchased from Wako Pure Chemical (Japan).

Microscopic Technique
Fluorescence Microscopy, Laser Scanning

Cell Type(s)
Neurons