Technical Reference #1534

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Mat Tek dishes (35 mm diameter sterile plastic Petri dishes with a no. 0 15 mm diameter glass coverslip glued onto the bottom; Mat Tek Corp.; model no. P35G-0-14-C)

1534.

Primary Culture Of Caenorhabditis Elegans Developing Embryo Cells For Electrophysiological; Cell Biological And Molecular Studies Kevin Strange; Michael Christensen; Rebecca Morrison, Vanderbilt University, Nature Protocols, 2(1534), (2007)
Link To Paper

Abstract:
Cell culture is an invaluable tool for investigation of basic biological processes. However technical hurdles including low cell yieldpoor cell differentiation and poor attachment to the growth substrate have limited the use of this tool for studies o

Materials & Methods:
Bacto Agar (Becton Dickinson and Co. catalog no. 214010) .Bacto Peptone (Becton Dickinson and Co. catalog no. 211677) .Bacto Tryptone (Becton Dickinson and Co. catalog no. 211705)

Microscopic Technique
Optical Microscopy, Differential Interference Contrast Microscopy, Fluorescence Microscopy

Cell Type(s)
NA22 Escherichia coli