Technical Reference #1533

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass Bottom Microwell Dishes (MatTek Corporation; www.glass-bottom-dishes.com)

1533.

Phagocytosis Of The Lyme Disease Spirochete; Borrelia Burgdorferi; By Cells From The Ticks; Ixodes Scapularis And Dermacentor Andersoni; Infected With An Endosymbiont; Rickettsia Peacockii Joshua T. Mattila; Ulrike G. Munderloh; Timothy J. Kurtii, University of Minnesota, Journal of Insect Science, 58(1533), (2007)
Link To Paper

Abstract:
Tick cell lines were used to model the effects of endosymbiont infection on phagocytic immuneresponses. The lines tested for their ability to phagocytose the Lyme disease spirochete Borreliaburgdorferi (Spirochaetales: Spirochaetaceae) were ISE6 and

Keywords:
coiling phagocytosis; tick cell culture; fluorescence microscopy; live cell imaging; vector competency

Materials & Methods:
Low passage B. burgdorferi isolate JMNT (Kurtti et al. 1988) and KS20 (Babb et al. 2004) a GFP-expressing strain of the B. burgdorferi isolate B31 were grown in Barbour-Stoenner-Kelly medium

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
IDE12